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. 2019 Jan 15;16:91–104. doi: 10.1016/j.omtn.2019.01.004

Figure 4.

Figure 4

miR-582-3p and miR-582-5p Repress TGF-β Signaling Activity in PCa Cells

(A–C) Upregulation of miR-582-3p (A), miR-582-5p (B), or both (C) attenuated transcriptional activity based on a TGF-β/Smad-responsive luciferase reporter in the absence or presence of TGF-β (5 ng/mL). *p < 0.05. (D) Western blot analysis showed that upregulation of miR-582-3p, miR-582-5p, or both decreased nuclear translocation of pSMAD3 in PCa cells in the absence or presence of TGF-β (5 ng/mL). The nuclear protein p84 was used as a nuclear protein marker. (E) Upregulation of miR-582-3p, miR-582-5p, or both reduced downstream bone metastasis-related genes of the TGF-β pathway, including CTGF, PTHRP, IL-11, NEDD9, MMP13, ADAM19, THBS1, COL1A1, and VEGFA, in the absence or presence of TGF-β (5 ng/mL). Transcript levels were normalized to GAPDH expression. Error bars represent the mean ± SD of three independent experiments.