Figure 4.

H3 Receptor Antagonism Promotes Neural Differentiation through H1R in NSCs following TBI

WT, Hrh1^fl/fl;Nestin^CreERT2, and Hrh2^fl/fl;Nestin^CreERT2 mice and their Nestin^CreERT2 littermates were treated with saline or H3R antagonist thioperamide (THIO; 10 mg/kg/day, i.p.) after cryogenic brain lesion. Confocal fluorescence microscopy images show longer process morphology of DCX⁺ or PSA-NCAM⁺ neuroblasts at 14 days after TBI in WT, Nestin^CreERT2, or Hrh1^fl/fl;Nestin^CreERT2 mice treated with thioperamide, but not in Hrh2^fl/fl;Nestin^CreERT2 mice (A and C). Image analysis of leading processes and total branch lengths shows greater lengths in THIO 10-treated neuroblasts in WT, Nestin^CreERT2, or Hrh1^fl/fl;Nestin^CreERT2 mice, but not in Hrh2^fl/fl;Nestin^CreERT2 mice using both DCX and PSA-NCAM staining (B and D). n = 5–6. ^#p < 0.05, ^##p < 0.01 versus the SALINE group of the same species. See also Figures S3 and S4.