Skip to main content
. 2019 Feb 7;12(3):461–473. doi: 10.1016/j.stemcr.2019.01.007

Figure 2.

Figure 2

Gene Expression Analyses of Treated SCs via Real-Time qPCR and Immunofluorescence

(A) Histogram view of the FACS purification of PAX7-GFP+ SCs and resulting pure population in phase contrast and GFP images.

(B) Real-time qPCR analysis of untreated (black bars) and DLL4 and PDGF-BB-treated (green bars) PAX7-GFP+ SCs. Graphs show fold change to control conditions, statistical significance based on ΔCt (N = 4).

(C) Left panel: PAX7-GFP+ SCs cultured in control (untreated) or DLL4 and PDGF-BB conditions and immunostained with MYOD (red) and PAX7 (green) antibodies. Right graph quantifying the relative percentages of PAX7/MYOD +/− cells in each condition (N = 3).

Data: means ± SEM. Statistical significance based on paired Student's t tests (B) or two-way ANOVA with Bonferroni's multiple comparison (C). ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗∗p ≤ 0.0001, n.s., not significant. Scale bars, 50 μm (A and C). See also Figure S1.