Figure 3.

In Vitro Assessment of Endothelial Network Association and Migration Properties of DLL4 and PDGF-BB-Treated SCs

(A) Representative images from endothelial network formation assays at 24 h with GFP+ untreated or DLL4 and PDGF-BB-treated SCs and control yellow fluorescent protein (YFP)+ primary muscle pericytes (technical control, from Tg:TN-AP-CreERT2:R26R-EYFP mice) stimulated with VEGF in the presence of HUVECs.

(B) Graph depicts quantification of network branches per mm² at 24 h in the same experimental groups shown in (A) (N = 4).

(C and D) Graph quantifies GFP+ SCs and YFP+ pericytes associated with the network per high-power field (HPF = 1.5 mm²) over time (N = 4); representative image of treated cells at 72 h shown in (D).

(E) Images of the lower side of transwell membranes from GFP+ untreated or treated SCs and control pericyte-derived mesoangioblasts (MABs) seeded on a monolayer of H5V murine endothelial cells. Graph quantifies the average number of cells/mm² that migrated through the endothelial layer after 6 h (N = 3).

Means ± SEM; statistical significance based on one-way ANOVA with Dunnett's multiple comparison test (B and C) or paired t test (E); ^∗p < 0.05, ^∗∗p ≤ 0.01. Scale bars, 100 μm.