Figure 2.

EP80317 inhibits NF-κB activation and production of inflammatory mediators in TsV-stimulated macrophages. (A–H) Peritoneal macrophages (PMs) were incubated or not with EP80317 (100 nM) for 2 h and later stimulated with TsV (50 μg/ml) for 24 h for quantification of cytokines/chemokine, or 5 min. for quantification of cAMP. (A) IL-1β, (B) IL-6, (C) TNF-α, (D) CCL3, (E) cAMP, (F) PGE₂, (G) LTB₄, and (H) IL-10. (I) PMs were pre-treated or not with EP80317 (100 nM) for 2 h and then stimulated with TsV (50 μg/ml). Cell lysates were obtained 2 h later for phospho-NF-κB p65 (Ser536) and total NF-κB p65 quantification. Data represent one of two independent experiments (n = 4 replicates). Differences were evaluated with one-way ANOVA followed by Bonferroni’s multi-comparison test. Data represent mean ± SDs, and significance is given by *p < 0.05, **p < 0.01, and ***p < 0.001.