Figure 1.

Cell-Laden Scaffold Assembly and Perfusion

(A and B) Fabrication of gelatin channel within supportive PDMS rig (A) and fully assembled perfusion platform (B).

(C) Schematic for cell seeding and initiation of experiments.

(D) BMECs stained with Calcein AM Ester following 7 days of culture on the channel surface as shown by orthogonal confocal image.

(E) Morphology comparison between Calcein AM-stained BMECs cultured in 2D tissue-culture plates (i and ii) and in gelatin channels (iii and iv).