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. 2019 Feb 14;12(3):474–487. doi: 10.1016/j.stemcr.2019.01.009

Figure 5.

Figure 5

Relative Impact of Shear on BMEC Permeability

(A) Permeability to 3 kDa dextran in IMR90-4-derived BMECs perfused at 100 μL/min over 21 days. N ≥ 3 independent biological replicates for all datasets, except N = 2 independent biological replicates for the day-21 time point. Diamonds indicate actual values of individual replicates. Error bars indicate ±1 SD.

(B) Permeability coefficients to 3 kDa dextran in BMECs perfused for 14 days at 100, 300, and 1,000 μL/min, compared with the static control. Diamonds indicate actual values of individual replicates. N = 2 independent biological replicates for 1,000 μL/min dataset, N ≥ 3 independent biological replicates for all other cohorts. Error bars indicates ±1 SD. ∗∗p < 0.01 compared with static based upon one-way ANOVA.

(C) Images showing cell sprouting in channels perfused for 14 days (left) at 100 and 1,000 μL/min, and 21 days (right) at 100 μL/min. Top image is bright-field, bottom image shows cells stained by rhodamine 123.