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. 2019 Feb 14;12(3):624–638. doi: 10.1016/j.stemcr.2019.01.010

Figure 2.

Figure 2

Lineage Tracing of SOX9+ Hepatocytes by Dural Recombination

(A) Schematic showing intersectional genetics for labeling of double-positive cells (SOX9+Hnf4a+).

(B) Schematic showing lineage-tracing strategy by Dre-rox and Cre-loxP recombinations. Dural recombination, but not singular one, labels SOX9+Hnf4a+ hepatocytes.

(C–E) Immunostaining for RFP, CK19, and HNF4a on liver sections shows periportal RFP+HNF4a+ hepatocytes (arrowheads) in Sox9-CreER;Hnf4a-DreER;R26-Ai66 (C) but not in Sox9-CreER;R26-Ai66 (D) or Hnf4a-DreER;R26-Ai66 (E) liver sections. Cartoon images on right panels show labeling result. Each figure is representative of five individual samples. Scale bars, 100 μm.