Figure 6.

Identification of Bipotent SOX9⁺ Hepatocyte after Liver Injury

(A and B) Fluorescent sections stained with BEC marker CK19 shows three distinct clones: hepatocyte clone, BEC clone, and mixed clone that contains both hepatocyte and BEC. Arrows or arrowheads indicate nGFP⁺, RFP⁺, or YFP⁺ hepatocytes or BECs, respectively. Cartoon images in (B) denote the magnified images in (A).

(C) Serial sections of a bipotent clone that contains both BECs (arrowheads) and hepatocytes (arrows).

(D) Quantification of the percentage of three types of clones in injured liver. A total of 246 clones were analyzed. Data are mean ± SEM; n = 5.

(E) Quantification of cell number in each type of clones. A total of 246 clones were analyzed. Data are mean ± SEM; n = 5.

(F) Cartoon figure showing the cell fate of three types of clones after injury.

Scale bars, 100 μm.