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. 2019 Jan 22;17(2):74. doi: 10.3390/md17020074

Figure 8.

Figure 8

MALDI-TOF-MS2 determines the acetylation pattern of partially acetylated COS. (A) The MS2 spectrum of A3D1 labelled with AMAC and the resulting ion fragments: A-amac, DA-amac, AA-amac, ADA-amac, DAA-amac, AAA-amac (m/z 438.15, 599.19, 641.20, 802.23, 844.24); so, the acetylation pattern of A3D1 is DAAA, ADAA and AADA. (B) MS2 spectrum of A2D2 labelled with AMAC and the resulting ion fragments: A-amac, DDA, DAD, ADD, DA-amac, AA-amac and ADA-amac, (m/z 438.16, 548.19, 599.22, 641.22, 802.29); so, the acetylation pattern of A2D2 is DDAA, ADDA and DADA. (C) MS2 spectrum of A1D3 labelled with AMAC, resulting in ion fragmentation of A-amac, DA-amac and DDA-amac (m/z 438.16, 599.21, 760.25); so, the acetylation pattern of A1D3 is DDDA. (D) The deacetylation process of ScCDA2 when A4 is used as a substrate.