Figure 6.

IFITMs do not interfere with CSFV binding but restrict CSFV entry. (A) Virus binding assay. PAMs were inoculated with CSFV at an MOI of 1 for 1 h at 4 °C. After washing with ice-cold phosphate-buffered saline (PBS), cell-bound CSFV was quantified by RT-qPCR. Data were normalized to β-actin expression. (B) Entry assay. PAMs were inoculated with CSFV at an MOI of 1 for 1 h at 4 °C. The viral inoculum was removed, and cells were washed with ice-cold PBS and then incubated with culture medium at 37 °C. After 2 h, PAMs were treated with PBS containing proteinase K to strip the CSFV that remained on the cell surface. CSFV genomic RNA levels were determined by RT-qPCR. Data were normalized to β-actin expression. Data represent the mean ± SD of three independent experiments and were measured in technical duplicate. Comparisons between groups were performed by Student’s t test. *** p < 0.001.