Table 1.
Primers used in this study.
| Primers | Sequence (5′–3′) | Purpose |
|---|---|---|
| CMV-IFITM1-F | CGGAATTCTATGATCAAGAGCCAGCACGA | Amplification of IFITM1 |
| CMV-IFITM1-R | CGGGATCCGTAGCCTCTGTTACTCTTTGCG | |
| CMV-IFITM2-F | CGGAATTCTATGAACTGCGCTTCCCAGC | Amplification of IFITM2 |
| CMV-IFITM2-R | CGGGATCCGTAGCCTCTGTTACTCTTTGCGC | |
| CMV-IFITM3-F | CGGAATTCTATGAATTGCGCTTCCCAGC | Amplification of IFITM3 |
| CMV-IFITM3-R | CGGGATCCGTAGCCTCTGTAATCCTTTATGAGCT | |
| CSFV-F | GAGAAGGACAGCAGAACTAAGC | RT-qPCR for detection of CSFV |
| CSFV-R | TTACCGCCCATGCCAATAGG | |
| β-actin-F | CAAGGACCTCTACGCCAACAC | RT-qPCR for detection of β-actin |
| β-actin-R | TGGAGGCGCGATGATCTT | |
| shN-F | GATCCGCTTAAACGCATAGTAGGACTCAAGAGAGTCCTACTATGCGTTTAAGCTTTTTG | Negative control of knockdown |
| shN-R | AATTCAAAAAGCTTAAACGCATAGTAGGACTCTCTTGAGTCCTACTATGCGTTTAAGCG | |
| shIFTIM-F | GATCCGCAAAGAGTAACAGAGGCTACCAAGAGGTAGCCTCTGTTACTCTTTGCTTTTTG | Knockdown of IFITMs |
| shIFITM-R | AATTCAAAAAGCAAAGAGTAACAGAGGCTACCTCTTGGTAGCCTCTGTTACTCTTTGCG | |
| IFITM1-F | TGGCTTTCGCCTACTCCG | RT-qPCR for detection of IFITM1 |
| IFITM1-R | ACAGTGGCTCCGATGGTCAG | |
| IFITM2/3-F | TCAACATCCGAAGCGAGACC | RT-qPCR for detection of IFITM2 and IFITM3 |
| IFITM2/3-R | GAGTAGGCGAAAGCCACGAA | |
| C1-IFITM1-F | CGGAATTCATGATCAAGAGCCAGCACGA | Amplification of IFITM1 |
| C1-IFITM1-R | CGGGATCCCTAGTAGCCTCTGTTACTCTTTGCG | |
| C1-IFITM2-F | CGGAATTCATGAACTGCGCTTCCCAGC | Amplification of IFITM2 |
| C1-IFITM2-R | CGGGATCCCTAGTAGCCTCTGTTACTCTTTGCGC | |
| C1-IFITM3-F | CGGAATTCATGAATTGCGCTTCCCAGC | Amplification of IFITM3 |
| C1-IFITM3-R | CGGGATCCCTAGTAGCCTCTGTAATCCTTTATGAGCT | |
| Mx1-F | TCTGTAAGCAGGAGACCATCAACT | RT-qPCR for detection |
| Mx1-R | TTTCTCGCCACGTCCACTATC | of Mx1 |
Restriction enzyme sequences underlined. Short hairpin sequences: loop in bold letters and Interference sequence in italics.