Table 1.
Primers | Sequence (5′–3′) | Purpose |
---|---|---|
CMV-IFITM1-F | CGGAATTCTATGATCAAGAGCCAGCACGA | Amplification of IFITM1 |
CMV-IFITM1-R | CGGGATCCGTAGCCTCTGTTACTCTTTGCG | |
CMV-IFITM2-F | CGGAATTCTATGAACTGCGCTTCCCAGC | Amplification of IFITM2 |
CMV-IFITM2-R | CGGGATCCGTAGCCTCTGTTACTCTTTGCGC | |
CMV-IFITM3-F | CGGAATTCTATGAATTGCGCTTCCCAGC | Amplification of IFITM3 |
CMV-IFITM3-R | CGGGATCCGTAGCCTCTGTAATCCTTTATGAGCT | |
CSFV-F | GAGAAGGACAGCAGAACTAAGC | RT-qPCR for detection of CSFV |
CSFV-R | TTACCGCCCATGCCAATAGG | |
β-actin-F | CAAGGACCTCTACGCCAACAC | RT-qPCR for detection of β-actin |
β-actin-R | TGGAGGCGCGATGATCTT | |
shN-F | GATCCGCTTAAACGCATAGTAGGACTCAAGAGAGTCCTACTATGCGTTTAAGCTTTTTG | Negative control of knockdown |
shN-R | AATTCAAAAAGCTTAAACGCATAGTAGGACTCTCTTGAGTCCTACTATGCGTTTAAGCG | |
shIFTIM-F | GATCCGCAAAGAGTAACAGAGGCTACCAAGAGGTAGCCTCTGTTACTCTTTGCTTTTTG | Knockdown of IFITMs |
shIFITM-R | AATTCAAAAAGCAAAGAGTAACAGAGGCTACCTCTTGGTAGCCTCTGTTACTCTTTGCG | |
IFITM1-F | TGGCTTTCGCCTACTCCG | RT-qPCR for detection of IFITM1 |
IFITM1-R | ACAGTGGCTCCGATGGTCAG | |
IFITM2/3-F | TCAACATCCGAAGCGAGACC | RT-qPCR for detection of IFITM2 and IFITM3 |
IFITM2/3-R | GAGTAGGCGAAAGCCACGAA | |
C1-IFITM1-F | CGGAATTCATGATCAAGAGCCAGCACGA | Amplification of IFITM1 |
C1-IFITM1-R | CGGGATCCCTAGTAGCCTCTGTTACTCTTTGCG | |
C1-IFITM2-F | CGGAATTCATGAACTGCGCTTCCCAGC | Amplification of IFITM2 |
C1-IFITM2-R | CGGGATCCCTAGTAGCCTCTGTTACTCTTTGCGC | |
C1-IFITM3-F | CGGAATTCATGAATTGCGCTTCCCAGC | Amplification of IFITM3 |
C1-IFITM3-R | CGGGATCCCTAGTAGCCTCTGTAATCCTTTATGAGCT | |
Mx1-F | TCTGTAAGCAGGAGACCATCAACT | RT-qPCR for detection |
Mx1-R | TTTCTCGCCACGTCCACTATC | of Mx1 |
Restriction enzyme sequences underlined. Short hairpin sequences: loop in bold letters and Interference sequence in italics.