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. 2019 Feb 11;11(2):104. doi: 10.3390/toxins11020104

Table 1.

Output statistics of de novo assembly of Naja sumatrana venom-gland transcriptome using Illumina HiSeq 2000 sequencing.

Parameter Sequencing Output
Total raw reads 47,494,560
Total clean reads 46,878,172
Contigs created 148,475
Q20 percentage 97.94%
N percentage 0.00%
GC percentage 44.16%
Unigenes/transcripts assembled 75,387
Number of transcripts (FPKM > 1) 55,386
Unidentified Abundance
Number of transcripts 35,449
Clean reads 123,432.1986
Total FPKM percentage (%) 7.95%
Non-toxin Abundance
Number of transcripts 19,877
Clean reads 199,393.4726
Total FPKM percentage (%) 12.84%
Toxin Abundance
Number of transcripts 60
Clean reads 1,230,548.6634
Total FPKM percentage (%) 79.22%