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. 2019 Jan 28;11(2):109. doi: 10.3390/v11020109

Figure 1.

Figure 1

Construction, expression, and purification of cell-penetrating Mx1-9R. Escherichia coli cells were transformed with pET28a vector containing Mx1-9R fusion DNA sequence. After isopropyl β-D-1-thiogalactopyranoside (IPTG) induction, expressed Mx1-9R were purified by Ni-NTA column. (A) Structure of the Mx1-9R fusion protein was designed as described in the Methods. (B) Agarose gel electrophoresis of amplified Mx1-9R insert. (C) SDS-PAGE (2%) was performed to confirm the induction of Mx1-9R. BL21 (DE3). Lane 1: Whole lysates of control cells cultured without IPTG; lane 2: Whole cells induced with IPTG; lane 3: Purified Mx1-9R using Ni-NTA column.