Figure 3.

Effect of Nm on translation elongation. (A) Western blot analysis of translation products carrying Nm at the codon 147 of the reporter mRNA and (B) at the second nucleotide of the AAU codon within the sequence context of the 5-HT_2cR mRNA. (C) Structure of the second base pair of the codon-anticodon interaction and the interaction with A1824 (A1492) of the 18S rRNA (16S rRNA) of the 40S (30S) ribosomal subunit. The eukaryotic decoding center is depicted in the foreground (modified from [54]), the E. coli decoding center in the background (modified from [55]).