Figure 1.

In vitro anti-viral activity of 25-HC on MNV replication. (A) RAW264.7 cells were infected with MNV at a multiplicity of infection (MOI) of approximately 0.05 TCID₅₀/cell and were immediately treated with a range of concentrations of 25-HC (up to 135 µM) or nystatin (up to 500 µM) or the ethanol-only control. At regular intervals, replicate wells were harvested, the supernatant clarified by centrifugation and the virus titer determined by TCID₅₀ assay. Data show mean virus titer (n = 2 ± SEM). (B) RAW264.7 cells were incubated with the indicated compounds for 72 h before the cell viability was measured by MTS assay. The ethanol (EtOH) or dimethyl sulphoxide (DMSO) solvents were used as controls. Data are expressed as mean percentage cell viability and normalized to untreated cells (n = 3 ± SEM).