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(A) Preparation of cationic niosome-based formulations containing lipids 11, 12 and 13, respectively and using SQ as a helper lipid; (B) in vitro gene transfection experiments after a 72-h incubation period at several mass ratios in three cell lines: HEK-293, ARPE-19 and PECC cells; (C) EGFP gene expression in retina after subretinal injection; (D) EGFP gene expression in the retina after intravitreal injection. Adapted with permission from ref. [82]. Copyright 2016, Elsevier B.V.
