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. 2019 Jan 25;11(2):100. doi: 10.3390/v11020100

Figure 5.

Figure 5

Effect of protein knockdown on HIV-1 mediated cell-cell fusion. (A) A depiction of the “fusion-from-without” (FFWO) assay that is based on detection of cell-cell fusion mediated by HIV-1 virions attached to adjacent cells. CoR, coreceptor. Cells are loaded with green (CMFDA) or orange/red (CMRA) cytoplasmic dyes and virus-mediated fusion between two adjacent cells is detected by appearance of double-labeled fusion products. (B) Representative images of fusion (FFWO) between scrambled shRNA-transduced CEM.CCR5 cells mediated by HXB2pp. Insert shows an enlarged view of the boxed area; the fused cells are marked by arrows. (C) Quantification of HXB2pp- and Bal26pp-mediated cell-cell fusion. The data points are mean and S.E. of 20 images from two independent experiments.