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. 2019 Feb 13;11(2):111. doi: 10.3390/toxins11020111

Figure 1.

Figure 1

The kinetics of fluorescence changes of PED6 incorporated in DOPC:PED6 8:2 LUV (data from Figure S1). The time 0 min corresponds to the addition of the following samples: buffer (light blue), urea (dark blue), BSA (green), Crotoxin (black), CyaAIP (orange), and CyaAUBC (red). (A) Increase of fluorescence emission intensity at 515 nm. (B) Ratio of fluorescence intensity at 515 nm, F515, at time t normalized by initial fluorescence at 515 nm. Inset shows same data with an expanded Y-axis (from 0.95 and 1.05). (C) Ratio of fluorescence intensity at 515 nm over fluorescence intensity at 575 nm: F515/F575. (D) The F515/F575 values are normalized to the initial F515/F575 value at 0 min (see methods for details).