Figure 3.

TGFβ-induced COL22A1 expression is inhibited by MEK and ALK5 inhibitors. Skin fibroblasts were incubated with 10 μmol/L SB431542 (SB), U0126 (U), LY294002 (LY), JNK inhibitor II (J), or DMSO as a vehicle control for 1 h before the addition of TGFβ (5 ng/mL). Skin fibroblasts were stimulated with TGFβ for 24 and 72 h. (a) Expression levels of COL22A1 were measured using qPCR (N = 4); ** p < 0.01. (b) Representative data from immunoblotting of lysates are shown. GAPDH was used as a loading control.