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. 2019 Feb 9;17(2):104. doi: 10.3390/md17020104

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of SA on small-intestinal injury in mice with MCD diet-induced steatohepatitis. (A) Histology was carried out using H&E staining (magnification, 100× and 200×), and representative images are shown. The height of five intact and well-oriented villi per sample was measured using ImageJ and the average value used as data. Results are the mean ± SEM for 4–6 mice per group. † p < 0.01. (B) Mucus production in the small intestine was detected using PAS staining (magnification, 100× and 200×), and representative images are shown. (C) Small-intestine sections were stained with ZO-1 (green) and DAPI (blue). Representative immunofluorescence images are shown. Magnified images represent high-magnification images of the boxed area.