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. 2019 Feb 21;17(2):128. doi: 10.3390/md17020128

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of the growth and protein content of BbMomL in different media and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). (A) Analysis of BbMomL growth in LB and MTNm. (B) Analysis of protein secretion by BbMomL in LB and MTNm. (C) The SDS-PAGE analysis of secretion of the target protein MomL at different culture time points. M: Marker; 1: Negative control; 2: 12 h; 3: 24 h; 4: 48 h; 5: 60 h; 6: 84 h; 7: 96 h; MomL: Positive control. The results shown are representative of biological duplicates. Error bars represent the standard deviations of three replicates.