Table 1.
List of primers used in RT-qPCR studies in mice. All primers have been validated to have greater than 95% of amplification efficiency. The expression levels of cytokine mRNA were normalized to the expression of the housekeeping gene HPRT. F: forward primer; R: reverse primer.
| Name | Sequence (5′-3′) |
|---|---|
| IFN-γ-F | TCAAGTGGCATAGATGTGGAAGAA |
| IFN-γ-R | TGGCTCTGCAGGATTTTCATG |
| IFN-α-F | CCTGTGTGATGCAACAGGTC |
| IFN-α-R | TCACTCCTCCTTGCTCAATC |
| IFN-β-F | ATCATGAACAACAGGTGGATCCTCC |
| IFN-β-R | TTCAAGTGGAGAGCAGTTGAG |
| IP-10-F | ATGACGGGCCAGTGAGAATG |
| IP-10-R | GAGGCTCTCTGCTGTCCATC |
| TNFα-F | AGGCACTCCCCCAAAAGATG |
| TNFα-R | CTGCCACAAGCAGGAATGAG |
| IL-1β-F | GTGTGGATCCCAAGCAATAC |
| IL-1β-R | GTCCTGACCACTGTTGTTTC |
| IL-18-F | TGGTTCCATGCTTTCTGGACTCCT |
| IL-18-R | TTCCTGGGCCAAGAGGAAGTGATT |
| IL-6-F | GTGGCTAAGGACCAAGACCA |
| IL-6-R | TAACGCACTAGGTTTGCCGA |
| IL-10-F | GCTGCCTGCTCTTACTGACT |
| IL-10-R | CTGGGAAGTGGGTGCAGTTA |
| RIG-I-F | CCTCCCATCTCCTTCATGACA |
| RIG-I-R | CCACCTACATCCTCAGCTACATGA |
| HPRT-F | GATTAGCGATGATGAACCAGGTT |
| HPRT-R | CCTCCCATCTCCTTCATGACA |