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. 2019 Jan 28;10(2):91. doi: 10.3390/genes10020091

Figure 3.

Figure 3

Complementation of ERF4 loss of function by ERF4-A or ERF4-R. Senescence phenotype and parameters were analyzed in Col-0, erf4 mutants, and erf4 mutant plants complemented with either ERF4-promoter:ERF4-A (cERF4-A) or ERF4-promoter:ERF4-R constructs (cERF4-R). (A) Automated colorimetric assay, in which rosette leaves are categorized according to color in four groups (green, green-yellow, yellow, brown). (B) Representative pictures of rosette leaves No. 1–10, which were sorted according to leaf age 55 DAS. (C) One representative picture of five biological replicates for DAB-staining in leaf No. 4 at 31–55 DAS. These plants have been raised under normal light intensities (80 μE/m2/s). (D) Fv/Fm values in rosette leaves were measured with pulse amplitude modulation (PAM). (E) Electrolyte Leakage (EL) was measured with a conductivity meter in detached leaves No. 4 of another experimental series. Values are normalized to fresh weight. Data A, D and E represent means of at least four biological replicates, error bars indicate ±SE.