Table 1.
Composition and characterization of the unfunctionalized ddMTX-loaded SLNs.
| Lipid Matrix | Stearic Acid | Behenic Acid | |
|---|---|---|---|
| Stabilizer | PVA9000 | PVA9000 | PVA120000 |
| Composition | |||
| Sodium stearate (mg) | 25 | - | - |
| Sodium behenate (mg) | - | 25 | 25 |
| PVA9000 (mg) | 25 | 50 | 50 1 |
| PVA120000 (mg) | - | - | 25 |
| 1 M Lactic acid (µL) | 125 | - | - |
| 1 M sodium hydroxide (µL) | - | 30 | 30 |
| 5 M ammonium chloride (µL) | - | 65 | 65 |
| 1 M hydrochloric acid (µL) | - | 100 | 100 |
| ddMTX (mg) | 2.0 | 2.0 | 2.0 |
| Distilled water (mL) | 2.5 | 2.5 | 1 2 |
| Characterization | |||
| Mean size (nm) | 353 ± 35 | 351 ± 45 | 435 ± 33 |
| Polydispersity | 0.240 | 0.155 | 0.250 |
| ddMTX (mg/mL) | 0.71 | 0.78 | 0.81 |
1 partially removed after centrifugation; 2 after centrifugation and resuspension in PVA120000 solution