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. 2019 Feb 13;11(2):154. doi: 10.3390/v11020154

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Secretory factors by PBMC samples of Ad-seropositive individuals contributed to promoting the efficacy of Ad infection. (A) Representative of paired PBMCs from Ad5-seronegative subjects and Ad5-seropositive subjects (n = 3 pairs) mixed together at different ratios. (B) The actual and calculated percentage of EGFP-positive cells in CD14+ cells of PBMC mixture after infected with 1250 vp/cell Ad5-EGFP. (C) The actual and calculated expression level of EGFP protein (represented with MFI value) in CD14+ cells of PBMC mixture after infected with 1250 vp/cell Ad5-EGFP. These data were analyzed statistically by nonparametric Mann-Whitney U test. The bars represent the standard error. *: p < 0.05.