Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 13;11(2):154. doi: 10.3390/v11020154

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Pre-existing virus-specific CD3+ T lymphocytes were more effectively activated after encountering adenovirus stimulus. (A) PBMCs of Ad-seropositive subjects (n = 15) or seronegative subjects (n = 5) were isolated, and the level of Ad-specific preexisting T lymphocytes in PBMCs was assessed ELISPOT assay as described in materials. (B–F) PBMCs of Ad-seropositive subjects (n = 3) or seronegative subjects (n = 3) were infected with 1250 vp/cell of recombinant Ad virus for 48 h, and the levels of cell proliferation (B), activation state including HLA-DR (C), CD38 (D), CD95 (E), CD27 (F), and cytokines/chemokines expression including IFN-γ (G), GM-CSF (H) and IL-4 (I) were further assessed as described in materials. The bars represent the standard error. *: p < 0.05, **: p < 0.01, ***: p < 0.001.