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. 2019 Feb 23;11(2):132. doi: 10.3390/toxins11020132

Figure 5.

Figure 5

Relative abundance of MUC2, MUC5AC and MUC5B protein in cell supernatants from Caco-2/HT29-MTX (A) 100/0, (B) 90/10, (C) 75/25, and (D) 0/100 co-cultures exposed to individual or combinations of AFM1 (0.05, 4 μg/mL), OTA (0.05, 4 μg/mL), or AFM1+OTA (0.05, 4 μg/mL) for 48 h. Relative levels of mucin proteins in the cell supernatant were measured by using human mucin 2, mucin 5 subtype B, and mucin 5 subtype AC ELISA kits. Results are expressed as a percentage of the control and are means ±SEM (n = 3). *, **, *** p < 0.05, 0.01, and 0.001, respectively, compared with control. M0.05 represents AFM1 at 0.05 μg/mL, M4 represents AFM1 at 4 μg/mL, O0.05 represents OTA at 0.05 μg/mL, O4 represents OTA at 4 μg/mL, M+O0.05 represents AFM1+OTA at 0.05 μg/mL, M+O4 represents AFM1+OTA at 4 μg/mL.