Table 2.
Comparison of kinetic rate constants for R-loop formation and DNA cleavage.
| Γ | k rloop,app a | k 1,app a | k 2,app a | |||
|---|---|---|---|---|---|---|
| SC | Figure 3 | Mg2+ | ~−7 pN nm | fixed as ≫ k1,app (τin < 5 s) | 0.203 ± 0.048 s−1 | 0.012 ± 0.004 s−1 |
| OC | Figure 3 | Mg2+ | 0 pN nm | 0.0067 ± 0.0037 s−1 (τin ≈ 149 s) | fixed as 0.203 s−1 | fixed as 0.012 s−1 |
| LIN1 | Figure 3 | Mg2+ | 0 pN nm | 0.0050 ± 0.0030 s−1 (τin ≈ 200 s) | fixed as 0.203 s−1 | fixed as 0.012 s−1 |
| LIN PCR | Figure 4C,D | Mg2+ | 0 pN nm | 0.0098 s−1 (τin ≈ 102 s) b | fixed as 0.203 s−1 | fixed as 0.012 s−1 |
a Errors = SD. b The same values were used for either the ordered or random simulation.