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. 2019 Feb 21;11(2):92. doi: 10.3390/pharmaceutics11020092

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The characterization of the M-MTX and M-MTX /siRNA complexes. (A) The particle size of M-MTX was measured by Zeta-sizer Nano ZS90. (B) SiRNA was then condensed by M-MTX with a different N/P ratio through the electrostatic interaction. (C) The particle size of the M-MTX/siRNA complexes at an N/P ratio of 16/1 was further determined.