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The internalization and endosome escape of M-MTX/siRNA in folate receptor (FR)-overexpressing HeLa cells: The internalization and endosome (red) escape of M-MTX loaded with FAM-siRNA (green) in the Hela cells was visualized by the confocal laser scanning microscopy (CLSM) at the first, second, and fourth hours. The red bar in the images was 20 μm.
