Figure 5.

Addition of the Flag-Sly transgene to males with NPYq deletions does not rescueX-Y gene upregulation. Gene expression in testes from NPYq-deficient mice without (9/10NPYq-) and with (9/10NPYq- tsgic) the Flag-Sly transgene was obtained by real-time RT-PCR with Actb as a loading control and normalized to wild-type XY controls. Two transgenic lines were analyzed: line 30A carrying the Sly1 transgene (A) and line 6P carrying both the Sly1 and Sly2 transgene (B). NPYq- deficient mice without the transgenes were negative siblings of transgenics. The graphs are mean ± SEM with n = 3. Statistical significance (t-test): * < 0.05; ** < 0.01; *** < 0.001, in comparison with NPYq- deficient mice with XY; the difference between transgenics and their negative siblings is marked with a horizontal lane above the relevant graph pair. Primer sequences are shown in Table S2.