Table 1.
Addition of the Flag-Sly transgene to males with severe NPYq deficiency does not rescue low sperm number and sperm ability to fertilize oocytes in vitro.
| Tg Line | Genotype | No Males | Age (Weeks) | Average ± SEM | Eggs Fertilized/Eggs Inseminated (%) | ||
|---|---|---|---|---|---|---|---|
| Body Weight (g) | Testis Weight (mg) | Sperm Number (1CE, ×106) | |||||
| 6P | 9/10NPYq-Sly1/2 | 4 | 10 | 27 ± 1 | 58 ± 4 | 0.1 ± 0.1 | 0/236 (0) |
| 9/10NPYq- | 3 | 10 | 26 ± 2 | 60 ± 3 | 1.9 ± 0.9 | 0/167 (0) | |
| WT IVF control | 2 | 16 | 34 ± 0 | 92 ± 3 | n/a | 75/121 (62) | |
| 16D | 9/10NPYq-Sly1 | 4 | 12 | 32 ± 4 | 79 ± 10 | 2.5 ± 1.2 | 1/236 (0) |
| 9/10NPYq- | 3 | 12 | 27 ± 1 | 81 ± 2 | 4.2 ± 2.4 | 0/166 (0) | |
| WT IVF control | 2 | 14 | 29 ± 1 | 94 ± 3 | n/a | 114/122 (93) | |
| 30A | 9/10NPYq-Sly1 | 3 | 10 | 26 ± 1 | 64 ± 4 | 0.4 ± 0.03 | 0/129 (0) |
| WT IVF control | 1 | 16 | 38 | 99, 100 | n/a | 39/49 (80) | |
Tg = transgene. 1CE = 1 cauda epididymis. There were no statistically significant differences between 9/10NPYq-Sly transgenics and their negative siblings 9/10NPYq- for any of the factors tested.