Figure 16.

In vivo therapeutic response to PPNs-mediated chemotherapy combined with phototherapy. (A) In vivo antitumor efficacy after the intravenous treatment of various DOX formulations combined with PPNs-mediated phototherapy (n = 6). The SKOV3 tumor-bearing mice were intravenously injected with PBS (control), free DOX (2.5 mg/kg), PPNs (2 mg/kg calculated on porphyrin), and PPNs–DOX (PPNs 2 mg/kg, DOX 2.5 mg/kg) on day 0, 5, 10, and 15, followed by various light irradiations for 2 min on tumors, 24 h post-injection. (B) Kaplan–Meier survival curves of SKOV3 tumor-bearing mice treated with the above indicated conditions, where tumor volume reached 500 mm³ and was considered as the end point of survival data. (C) Photographs showing therapeutic response to PPNs-mediated phototherapy with irradiation at 0.5 W/cm² for 2 min and 1.25 W/cm² for 2 min, respectively. (D) H&E staining of tumor sections collected from control mice and PPNs-injected (2 mg/kg) and variously irradiated mice (0.5 W for 2 min and 1.25 W for 2 min, respectively), 24 h post-injection. Reproduced from Reference [54] (Theranostics 07:3901 image No. 005, open access policy).