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. 2019 Feb 1;10(2):39. doi: 10.3390/insects10020039

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Correlation between CHIKV titers of saliva and salivary glands. The presence of a salivary gland escape barrier (SGEB) was tested by correlating CHIKV titers in salivary glands with virus titers in saliva. Saliva was collected at 9 days post infection and then salivary glands were dissected from the same mosquitoes. Virus titers from salivary glands and saliva were determined by plaque assays. (A) Correlation between saliva and salivary gland of Ae. aegypti (Poza Rica) infected with CHIKV R99659 (●) and CHIKV LR2006 OPY1 (■). (B) Correlation between saliva and salivary gland of Ae. albopictus infected with CHIKV R99659. Florida strain (●), New Jersey strain (■), Louisiana, (▼) and La Réunion (♦). (C) Correlation between saliva and salivary gland of Ae. albopictus infected with CHIKV LR2006 OPY1. Florida strain (●), New Jersey strain (■), Louisiana, (▼) and La Réunion (♦). The % SGEB is the number of saliva samples without virus divided by the number of virus positive salivary gland samples. Percentage of mosquitoes with SGEB are listed for each group.