Table 2.
Screening method performances—comparison with previous validation studies.
| Mycotoxin | Present Training Course | Previous Studies | ||||
|---|---|---|---|---|---|---|
| Validation Parameters | Study Design | Guidelines | Validation Parameters | Reference | ||
| DON | FPIA/ wheat |
STC: 1600 µg/kg intermediate precision TC: 10% repeatability: 10% cut-off: 1504 µg/kg false suspects for blanks: <0.1% |
single laboratory | CEN/TR 13505 | STC: 1750 µg/kg recovery: 97% repeatability: 4.1% limit of detection: 80 µg/kg |
Lippolis et al. [15] |
| ELISA/ wheat |
STC: 1600 µg/kg intermediate precision TC: 30% repeatability: 21% cut-off: 670 µg/kg false suspects for blanks: <0.1% |
single laboratory | AOAC performance tested | STC: 1000 µg/kg recovery: 100% repeatability: 22% limit of quantification: 167 µg/kg |
r-biopharm [16] | |
| LFD/ wheat |
STC: 1600 µg/kg intermediate precision TC: 6.5% repeatability: 4.8% cut-off: 980 µg/kg false suspects for blanks: <0.1% |
single laboratory | 519/2014/EC | STC: 1600 µg/kg intermediate precision: 13% repeatability: 12% cut-off: 1410 µg/kg false suspects for blanks: <0.1% |
unpublished results | |
| LC-HRMS/ wheat |
STC: 1600 µg/kg intermediate precision TC: 10% repeatability: 8.4% cut-off: 1151 µg/kg relative cut off: 0.71 false suspects for blanks: <0.1% |
inter-laboratory | 519/2014/EC | STC: 250 µg/kg reproducibility: 11% repeatability: 3.4% cut-off: 184 µg/kg relative cut off: 0.74 false suspects for blanks: <0.1% |
prEN 17279:2018 [17] | |
| AFB1 | LFD/ maize |
STC: 10.6 µg/kg intermediate precision TC: 16% repeatability: 7.2% cut-off: 6.4 µg/kg false suspects for blanks: <0.1% |
single laboratory | 519/2014/EC | STC: 4 µg/kg intermediate precision*: 29% repeatability: 21% cut-off: 2.18 µg/kg false suspects for blanks: 8% |
Lattanzio et al. [18] |
| LC-HRMS/ wheat |
STC: 2 µg/kg intermediate precision TC: 33% repeatability: 28% cut-off: 1.23 µg/kg false suspects for blanks: <0.1% |
inter-laboratory | 519/2014/EC | STC: 2 µg/kg reproducibility: 25% repeatability: 19% cut-off: 1.08 µg/kg false suspects for blanks: <0.1% |
prEN 17279:2018 [17] | |
* Sum of repeatability + day-to-day variation + matrix-to-matrix variation.TC: training course intermediate precision: repeatability + within technician (sample preparation) variation.