Table 2.

Summary of EBOV/Mak isolation success using three cell lines and suckling BALB/c laboratory mice.

Target PFU/Sample	EBOV/Mak Virus Isolation by Method
	Vero E6				Huh-7					MDM				Mice
	1	2	3	4	1	2	3	4	5	1	2	3	4	4 b	5 c
100	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
10	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
1	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
0.1 d	-	+	-	+	+	+	- a	+	+	-	+	+	+	+	+
0.01 d	-	-	-	-	-	-	+ a	-	-	-	-	-	-	-	-
0.001 d	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

^a These samples were retested and results were confirmed. ^b Brain tissue harvested at day 10 from all animals. ^c Brain tissue harvested when animal succumbed to disease or on day 28 post-exposure (scheduled necropsy). ^d Plaque assay titers for these samples are below the lower limit of quantification. Abbreviations: “+” indicates successfully virus isolation attempt; “-” indicates unsuccessful virus isolation attempt.