Table 4.
Target PFU/Sample | Virus Isolation Success in Spiked Samples by Cell Type a (Total Positive/12 Tested) |
||||||||
---|---|---|---|---|---|---|---|---|---|
Semen | Breast Milk | Media | |||||||
VeroE6 | Huh-7 | MDM | VeroE6 | Huh-7 | MDM | VeroE6 | Huh-7 | MDM | |
100 | 12 | 12 | 12 | 0 | 0 | 0 | 12 | 12 | 10 |
10 | 12 | 12 | 10 | 0 | 0 | 0 | 12 | 11 | 11 |
1 | 12 | 11 | 5 | 0 | 0 | 0 | 12 | 11 | 8 |
0.1 | 7 | 8 | 0 | 0 | 0 | 0 | 2 | 3 | 1 |
0.01 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 |
0.001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
a Isolation attempt completed in individual wells of 6 well plate (9 cm2/well). Sample volume added was 10 µL sample/well.