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. 2019 Feb 11;11(2):149. doi: 10.3390/v11020149

Figure 3.

Figure 3

Amino acid substitutions potentially involved in evasion from neutralizing mAbs. (A) Alignment of H7-HA sequences. Amino acid sequences of HA derived from the human H7N9 viruses tested in Table 5 were aligned. Since all tested mAbs targeted the HA head, the HA head sequences are shown. Each colored circle indicates each position on the HA structure. (B) Amino acid substitution sites mapped onto the H7-HA molecule. Amino acid mutations that were identified from escape mutant viruses (A135V, G144E, and L226Q) and substitutions that appear to be important for evasion from mAb recognition (I130T, T132A, A135T, and R140K) were mapped onto the three-dimensional (3D) structure of the H7-HA trimer (PDB; 4LCX) by using the molecular graphics system PyMOL. Cyan indicates the receptor binding site.