. 2019 Feb 8;9(2):233. doi: 10.3390/nano9020233

Table 1.

Commonly used fluorogenic RNA complexes and their spectral properties.

Aptamer	Fluorophore	K_D (nM)	Ex./Em. (nm)	Ɛ (M⁻¹cm⁻¹) ^a	ɸ ^b	Brightness ^c
Spinach [35]	DFHBI	540	469/501	24,300	0.72	100
Spinach2 [31]	DFHBI	530	447/501	22,000	0.72	91
Spinach2 [31]	DFHBI-1T	560	482/505	31,000	0.94	167
Spinach2 [31]	DFHBI-2T	1300	500/523	29,000	0.12	20
Broccoli [79]	DFHBI-1T	360	472/507	29,600	0.94	159
Corn [83]	DFHO	70	505/545	29,000	0.25	41
Mango [84]	TO1-Biotin	3.2	510/535	77,500	0.14	62
Mango [84]	TO3-Biotin	5.1	637/658	9300	N/A ^d	N/A
Mango II [85]	TO1-Biotin	0.7	510/535	77,000	0.2	88
Mango III [85]	TO1-Biotin	5.6	510/535	77,000	0.56	247
Mango IV [85]	TO1-Biotin	11.1	510/535	77,000	0.42	185
DNB [87]	SR-DN	800	572/591	50,250	0.98	282
SRB-2 [86]	SR-DN	1400	579/596	85,200	0.65	317

^a Ɛ—Extinction Coefficient. ^b ɸ—Quantum Yield. ^c Brightness—Extinction Coefficient × Quantum Yield relative to Spinach-DFHBI. ^d N/A—Not Available.