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. 2019 Jan 31;11(2):128. doi: 10.3390/v11020128

Figure 2.

Figure 2

PIV5 M protein binds to AMOTL1, but not to AMOT or AMOTL2. 293T cells were transfected to produce PIV5 M protein together with the indicated Flag-tagged angiomotins. Proteins synthesized in the transfected cells were metabolically labeled with 35S, and cells were lysed in a solution containing 0.5% NP-40. Immunoprecipitation was carried out using a combination of anti-Flag and anti-PIV5 M antibodies (left), or using Flag antibody only (right). Proteins were fractionated on SDS gels and detected using a phosphorimager. Boxed region denotes M co-precipitation.