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. 2019 Jan 29;10(2):96. doi: 10.3390/genes10020096

Figure 3.

Figure 3

mTOR-sensitivity of β-actin mRNA translation is cell type-dependent. HeLa, PC3 or SK-N-SH cells were transfected with pNL2.2. After 2h of 500 nM Torin1 treatment (A) or amino acid and serum starvation, -AA (B) the cells were lysed, and luciferase activity was measured. Student’s t-test was used to estimate the statistical significance versus SLU7. (C) HEK293T, HeLa, PC3 or SK-N-SH cells were treated for 2h with DMSO (control), 500 nM Torin1, or subjected to amino acid and serum starvation (-AA). Cells were lysed and analyzed by Western blotting using the indicated antibodies. *** p < 0.001; #—nonsignificant.