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. 2019 Jan 29;10(2):96. doi: 10.3390/genes10020096

Figure 4.

Figure 4

TSS activity in the studied cell types. (A–B) HEK293T, HeLa, PC3 or SK-N-SH cells were transfected with pNL2.2-ACTB (A) or pNL2.2-RPL32 (B). After 24 h, the total RNA was extracted and 5’ends of the reporter mRNAs were analyzed by 5’RACE. HeliscopeCAGE signal [32] in the vicinity of ACTB or RPL32 transcription start sites in HEK293, HeLa, PC3 cells and the average signal across all FANTOM5 cell types are presented. The TATA-box is shown in blue, putative TOP [22] (ACTB) and TOP (RPL32) are shown in red.