Table 2.
List of primers used for quantitative real-time polymerase chain reaction for analysis of apoptotic gene expression.
| Gene | Primer | TM (°C) |
|---|---|---|
| P53 | F:AGAGACCGTACAGAAGA | 58 |
| R:CTGTAGCATGGGATCCTTT | ||
| P21 | F:GTTGCTGTCCGGACTACCG | 53 |
| R:AAAAACAATGCCACCACTCC | ||
| Caspase-3 | F:AGGGGTCATTTATGGGACA | 58 |
| R:TACACGGGATCTGTTTCTTTG | ||
| R:CAGGCCTGGATGAAGAAGAG | ||
| Bax | F:CGAGCTGATCAGAACCATCA | 58 |
| R:GAAAAATGCCTTTCCCCTTC | ||
| R:AACCATACTCGAACCACATCCT | ||
| Bcl-2 | F:TAAGCTGTCACAGAGGGGCT | 58 |
| R:TGAAGAGTTCCTCCACCACC | ||
| R:AAAGGAGGCTACACCCCAGT | ||
| Bak | F: CTC AGA GTT CCA GAC CAT GTT G | 58 |
| R: CAT GCT GGT AGA CGT GTA GGG | ||
| R:CCTTTGTACCGTTGCATCCT | ||
| GAPDH | F:AGGTCGGTGTGAACGGATTTG | 58 |