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Validation and expression of eight selected genes detected by real time-quantitative PCR (RT-qPCR). TaFBR1, 2, 3: Flavonoid biosynthesis related gene1, 2, 3. HSP70-1: the gene encoding heat shock protein 70. GS1, GS2: the genes encoding glutathione S-transferase. F3-1: flavonoid 3′-monooxygenase. Data represents the mean ± SD calculated from three independent replicates. Gene expression levels were normalized to the internal control TaActin.
