Figure 4.

Effect of gukulenin A on the induction of apoptosis in human ovarian cancer cells. A2780 cells were treated with the indicated concentration of gukulenin A (15, 30, and 60 nM) for 48 h and then double stained with PI and Annexin V-FITC. (A) Flow cytometry analysis was performed for the staining profiles of the cells. The data are representative of three independent experiments. (B) The respective cell percentages in early and late apoptosis are presented in the bar graph. The values shown are the mean of three independent experiments. * p < 0.05 as compared with the untreated group.