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. 2019 Feb 21;17(2):126. doi: 10.3390/md17020126

Figure 5.

Figure 5

Involvement of caspases in gukulenin A-induced apoptosis in human ovarian cancer cells. (A) The effect of gukulenin A on caspase activation in human ovarian cancer cells. After 48h treatment of A2780 cells with gukulenin A (15, 30, and 60 nM), Western blot assay was performed to determine the levels of pro/cleaved caspase-3, -8, and -9. β-Actin was used as an internal control. The immunoblots are representative of three independent experiments. (B) The effect of caspase inhibitors on gukulenin A-induced cell death in human ovarian cancer cells. A2780 cells were treated with gukulenin A (60 nM) in the presence of caspase-3 inhibitor z-DEVD-fmk (50 μM), caspase-8 inhibitor z-IETD-fmk (50 μM), caspase-9 inhibitor z-LEHD-fmk (50 μM), and broad caspase inhibitor z-VAD-fmk (50 μM) for 24 h. An MTT assay was performed to assess the cell death. * p < 0.05 as compared with the untreated group. # p < 0.05 as compared with the gukulenin A only treated group.