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. 2019 Feb 18;10(2):155. doi: 10.3390/genes10020155

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Real-time quantitative polymerase chain reaction (qRT-PCR) verification of DEGs detected by gene expression profiling. The expression levels of DEGs related to lipid metabolism determined by qRT-PCR in the ovariectomized and sham-operated groups. These genes were involved in glycerolipid metabolism, sterol biosynthesis, and fatty acid biosynthesis, among others. Each of these DEGs were up-regulated or down-regulated significantly (p < 0.05) in the ovariectomized group compared with the sham-operated group.