Table 1.

Overview of publications on jellyfish-associated microbiome in terms of species studied (and their taxonomy) and jellyfish life stage, body compartment, sampling location, and methodology applied to analyze the composition and/or structure of the associated microbiome. FISH, fluorescence in situ hybridization; DGGE, denaturing gradient gel electrophoresis; ARISA, automated ribosomal intergenic spacer analysis; ITS, internal transcribed spacer; NGS, next-generation sequencing; T-RFLP, terminal restriction fragment length polymorphism.

Jellyfish Taxonomy					Study Design
Phylum/Subphylum	Class	Order	Family	Species	Life Stage	Body Part (Adult Medusae)	Sampling Location	Methodology to Study Associated Microbiota	Publication
Medusozoa	Scyphozoa	Semaeostomeae	Ulmaridae	Aurelia aurita	Adult medusae	Whole body	North Atlantic coastal waters	16S rRNA gene clone libraries	[36]
					Polyps, strobila, ephyra, juvenile adult medusae	Mucus, gastric cavity	Kiel Bight, Baltic Sea, Southern English Channel, North Sea	Confocal laser scanning microscopy FISH NGS 454 technology V1–V2 16S rRNA region	[35]
					Adult medusae	Oral arms umbrella gastric cavity	Northern Adriatic	Culturing, DGGE, 16S rRNA gene clone libraries	[37]
			Cyaneidae	Cyanea capillata	Adult medusae	Tentacles	Scottish waters (Orkney)	Culturing DGGE, sequencing bands	[38]
				Cyanea lamarckii	Adult medusae	Tentacles	Scottish waters (Orkney)	Culturing DGGE, sequencing bands	[38]
					Larvae polyps adult medusae	Tentacles, umbrella, mouth arm, gonads	German Bight	ARISA of ITS region	[39]
			Pelagiidae	Pelagia noctiluca	Adult medusae	Mouth	Ireland	Sequencing of specific bacterial 16S rRNA gene	[33]
				Chrysaora plocamia	Polyps podocyst excyst	Whole body	Northern Chile	NGS Illumina MiSeq platform 2 × 300 bp paired end, V1–V2 16S rRNA region	[40]
				Chrysaora hysoscella	Larvae polyps adult medusae	Tentacles, umbrella, mouth arm, gonads	German Bight	ARISA of ITS region	[39]
		Rhizostomeae	Mastigiidae	Mastigias cf. papua	Adult medusae	Dome, tentacles	Indonesian marine lakes	NGS 454 technology, V3–V4 region	[41]
			Cepheidae	Cotylorhiza tuberculata	Adult medusae	Gastric cavity	Alcudia Bay, Balearic Sea	Culturing, NGS—454 pyrosequencing	[42]
					Adult medusae	Gastric cavity	Alcudia Bay, Balearic Sea	NGS—Illumina MiSeq platform, 2 × 250 bp, paired end	[43]
	Cubozoa	Carybdeida	Tripedaliidae	Tripedalia cf. cystophora	Adult medusae	Whole body	Indonesian marine lakes	NGS—454 technology V3-V4 16S rRNA region	[41]
	Hydrozoa	Anthoathecata	Bougainvilliidae	Nemopsis bachei	Adult medusae	Whole body	North Atlantic coastal waters	16S rRNA gene clone libraries	[36]
			Tubulariidae	Tubularia indivisa	Adult medusae	Tentacles	Scottish waters (Orkney)	Culturing DGGE, sequencing bands	[38]
		Leptothecata	Phialellidae	Phialella quadrata	Adult medusae	Whole body	Shetland Isles	Nested PCR with specific bacterial primers	[32]
					Adult medusae	Whole body	Ireland	RT PCR with specific bacterial primers	[34]
		Siphonophorae	Diphyidae	Muggiaea atlantica	Adult medusae	Whole body	Ireland	RT PCR with specific bacterial primers	[34]
Ctenophora	Tentaculata	Lobata	Bolinopsidae	Mnemiopsis leidyi	Adult specimen	Whole body	Tampa Bay, Florida, USA	16S rRNA gene clone libraries, T-RFLP	[44]
					Adult specimen	Whole body guts	Gullmar fjord, west coast of Sweden	NGS—454 pyrosequencing	[23]
					Adult specimen	Whole body	Helgoland roads, German Bight	ARISA of ITS region	[45]
				Bolinopsis infundibulum	Adult specimen	Whole body	Helgoland roads, German Bight	ARISA of ITS region	[45]
		Cydippida	Pleurobrachiidae	Pleurobrachia pileus	Adult specimen	Whole body	Helgoland roads, German Bight	ARISA of ITS region	[45]
	Nuda	Beroida	Beroidae	Beroe ovata	Adult specimen	Whole body	Helgoland roads, German Bight	ARISA of ITS region	[45]
					Adult specimen	Whole body	Tampa Bay, Florida, USA	16S rRNA gene clone libraries, T-RFLP	[44]