Fig. 6.

Effect of the treatment with 16-hydroxy betulinic acid (50, 100 mg/kg) and morphine as control (10 mg/kg) on the hot plate analgesic test. Each mouse is served as its own control. Before treatment, the time for hind paw licking or jumping on the heated plate of analgesiometer was determined thrice at 1 h interval and mean of these were taken as the initial reaction time (control latency). Mice in each group were tested 30, 60 and 90 min after drug treatment. Values are expressed as mean ± SEM, ^∗P < 0.05 and ^∗∗P < 0.01 compared with control group.